Mouse CORT (Corticosterone) ELISA Kit (AEES00019)
- SKU:
- AEES00019
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 1.93 ng/mL
- Range:
- 3.12-200 ng/mL
- Sample Type:
- Serum and plasma
Description
Product Name: | Mouse CORT (Corticosterone) ELISA Kit |
SKU: | AEES00019 |
Target: | Mouse CORT (Corticosterone) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 1.93 ng/mL |
Detection range: | 3.12-200 ng/mL |
Kit component: |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum and plasma; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of CORT concentrations in serum and plasma. |
Specificity: | This kit recognizes CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed. |