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Mouse Complement Component 5a Receptor 1 (C5aR1) ELISA Kit

SKU:
MODL00185
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P30993
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD88,C5A,C5-AR1,C5R1,C5a anaphylatoxin chemotactic receptor 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Complement Component 5a Receptor 1 (C5aR1) ELISA Kit

The Mouse Complement Component 5a Receptor 1 (C5AR1) ELISA Kit is specifically designed for the precise measurement of C5AR1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.C5AR1 is a critical receptor involved in the immune response, particularly in the activation of inflammatory reactions and recruitment of immune cells.

Dysregulation of C5AR1 has been implicated in various inflammatory diseases, making it a key target for therapeutic interventions.By using the Mouse C5AR1 ELISA Kit, researchers can gain valuable insights into the role of C5AR1 in immune responses and inflammatory conditions, ultimately leading to a better understanding of disease mechanisms and the development of novel treatments.

Product Name:Mouse Complement Component 5a Receptor 1 (C5aR1) ELISA Kit
Product Code:MODL00185
Size:96 Assays
Target:C5aR1
Synonyms:CD88, C5A, C5-AR1, C5R1, C5a anaphylatoxin chemotactic receptor 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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