The Mouse Complement Component 4b (C4b) ELISA Kit is a powerful tool for the precise measurement of C4b levels in mouse serum, plasma, and tissue homogenates. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable asset for a variety of research applications.C4b is a key component of the complement system, playing a critical role in immune response and inflammation. Dysregulation of C4b has been implicated in a range of diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions.
By accurately measuring C4b levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.The Mouse Complement Component 4b (C4b) ELISA Kit is a valuable tool for researchers studying the immune system, inflammation, and related pathologies in mouse models. With its high sensitivity and reliability, this kit enables precise quantification of C4b levels, facilitating in-depth research and the development of novel treatment strategies.
Product Name:
Mouse Complement Component 4b (C4b) ELISA Kit
Product Code:
MODL00182
Size:
96 Assays
Target:
C4b
Synonyms:
CO4, CPAMD3, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-103
97
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
