The Mouse Col4 (Collagen Type IV) ELISA Kit is specifically designed for the precise measurement of Col4 levels in mouse biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Collagen Type IV is a key component of the extracellular matrix, playing a crucial role in maintaining tissue structure and function. Dysregulation of Col4 has been implicated in various diseases such as kidney disorders, vascular diseases, and certain cancers.
As such, this ELISA kit is an essential tool for studying the role of Col4 in disease pathogenesis and for developing potential therapeutic interventions.Overall, the Mouse Col4 (Collagen Type IV) ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for measuring Col4 levels in mouse samples, making it an invaluable asset for advancing our understanding of extracellular matrix biology and associated disease states.
Product Name:
Mouse COL4 (Collagen Type IV) ELISA Kit
SKU:
MOES00881
Target:
Mouse COL4 (Collagen Type IV)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL4. You can calculate the concentration of Mouse COL4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
87-101
97-111
Average (%)
102
92
103
1:4
Range (%)
91-102
82-94
81-96
Average (%)
97
88
88
1:8
Range (%)
91-105
80-93
82-95
Average (%)
97
87
88
1:16
Range (%)
90-100
82-95
88-102
Average (%)
95
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.88
10.15
35.33
4.75
10.94
37.49
Standard deviation
0.25
0.54
1.26
0.28
0.52
1.6
C V (%)
5.12
5.32
3.57
5.89
4.75
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse COL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL4 in samples. No significant cross-reactivity or interference between Mouse COL4 and analogues was observed.
