The Mouse CNP (C-Type Natriuretic Peptide) ELISA Kit is specifically designed for the precise measurement of CNP levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it an excellent tool for various research applications.C-Type Natriuretic Peptide is a key player in regulating blood pressure and cardiovascular function, making it a significant biomarker in cardiovascular diseases and hypertension.
By accurately measuring CNP levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic strategies.Overall, the Mouse CNP ELISA Kit from www.assaygenie.com provides researchers with a valuable tool for studying the role of C-Type Natriuretic Peptide in various physiological and pathological processes, paving the way for advancements in cardiovascular research and drug development.
Product Name:
Mouse CNP (C-type natriuretic peptide) ELISA Kit
SKU:
MOES00926
Target:
Mouse CNP (C-type natriuretic peptide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CNP. During the reaction, CNP in the sample or standard competes with a fixed amount of CNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
91-104
98-115
Average (%)
106
98
105
1:4
Range (%)
85-98
93-105
99-112
Average (%)
92
99
106
1:8
Range (%)
85-99
91-105
96-111
Average (%)
90
97
102
1:16
Range (%)
85-100
91-106
93-105
Average (%)
91
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.2
150.1
345.4
54.1
162.3
320.1
Standard deviation
2.9
8.0
16.9
3.8
9.1
14.4
C V (%)
5.66
5.33
4.89
7.02
5.61
4.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CNP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes CNP in samples. No significant cross-reactivity or interference between CNP and analogues was observed.
