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Mouse Cluster Of Differentiation 83 (CD83) ELISA Kit

SKU:
MODL00240
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88324
Sensitivity:
35pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
BL11,HB15,Activated B Lymphocytes,Immunoglobulin,B-cell activation protein,Cell surface protein HB15
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Cluster Of Differentiation 83 (CD83) ELISA Kit

The Mouse Cluster of Differentiation 83 (CD83) ELISA Kit is specifically designed for the quantitative detection of CD83 levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.CD83 is a cell surface protein involved in regulating immune responses and is known to play a role in T cell activation and tolerance.

Its expression is crucial for immune system function and has been linked to various autoimmune diseases, making it a valuable biomarker for studying immune responses and developing potential therapies.Overall, the Mouse CD83 ELISA Kit provides researchers with a powerful tool for investigating the role of CD83 in immune function and disease pathogenesis in mouse models.

Product Name:Mouse Cluster Of Differentiation 83 (CD83) ELISA Kit
Product Code:MODL00240
Size:96 Assays
Target:CD83
Synonyms:BL11, HB15, Activated B Lymphocytes,Immunoglobulin, B-cell activation protein, Cell surface protein HB15
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)84-10293
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)92-9895
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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