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Mouse Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

SKU:
MODL00237
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27548
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD154,TRAP,HIGM1,IGM,IMD3,TBAM,T-BAM,TNFSF5,Gp39,TNF Superfamily Member 5,Hyper-IgM Syndrome,TNF-Related Activation Protein,T-Cell B-Cell Activating Molecule
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

The Mouse Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of CD40L levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.CD40L is a key protein involved in immune response modulation and inflammation regulation. It plays a crucial role in various immune-related diseases, including autoimmune disorders and inflammatory conditions.

Understanding CD40L levels can provide valuable insights into the mechanisms of these diseases and potential therapeutic targets.With its high-quality components and user-friendly protocol, the Mouse CD40L ELISA Kit from Assaygenie is a valuable tool for researchers studying immune response, inflammation, and related diseases in mouse models. Trust in the accuracy and precision of this kit to advance your research efforts.

Product Name:Mouse Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit
Product Code:MODL00237
Size:96 Assays
Target:CD40L
Synonyms:CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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