The Mouse CKM (Creatine Kinase-Muscle) ELISA Kit is specifically designed for the accurate and reliable detection of CKM levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CKM is an important enzyme found predominantly in muscle tissue, playing a key role in energy metabolism and muscle contraction.
Elevated CKM levels can indicate muscle damage or disease, making it a valuable biomarker for studying muscular disorders, exercise-induced muscle damage, and monitoring the effects of potential treatments.With this Mouse CKM ELISA Kit, researchers can confidently measure CKM levels in mouse samples with ease and precision, enhancing their understanding of muscle physiology and pathology.
Product Name:
Mouse CKM (Creatine Kinase, Muscle) ELISA Kit
SKU:
MOES00917
Target:
Mouse CKM (Creatine Kinase, Muscle)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CKM. You can calculate the concentration of Mouse CKM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
87-102
97-111
Average (%)
99
94
104
1:4
Range (%)
87-100
85-95
83-96
Average (%)
94
90
90
1:8
Range (%)
91-106
85-100
90-102
Average (%)
97
91
95
1:16
Range (%)
87-101
78-92
87-99
Average (%)
93
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
86-102
93
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.84
8.58
34.85
4.54
9.21
32.54
Standard deviation
0.26
0.38
1.75
0.23
0.55
1.1
C V (%)
5.37
4.43
5.02
5.07
5.97
3.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CKM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CKM in samples. No significant cross-reactivity or interference between Mouse CKM and analogues was observed.
