The Mouse CKM (Creatine Kinase-Muscle) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of CKM levels in mouse serum, plasma, and cell lysates. This kit provides reliable and reproducible results, making it an essential tool for researchers studying muscle function and disease processes.CKM is an important enzyme found in muscle tissue that plays a key role in energy production and muscle metabolism. Abnormal levels of CKM have been associated with various muscle disorders, making it a valuable biomarker for monitoring muscle injury and disease progression.
With its user-friendly protocol and quick results, the Mouse CKM CLIA Kit is ideal for a wide range of research applications, including studies on muscle physiology, exercise physiology, and muscle-related pathologies. Get accurate and reliable data with this advanced assay kit from Assay Genie.
Product Name:
Mouse CKM (Creatine Kinase, Muscle) CLIA Kit
SKU:
MOES00204
Target:
Mouse CKM (Creatine Kinase, Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CKM. You can calculate the concentration of Mouse CKM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
99-114
91-103
Average (%)
106
105
97
1:4
Range (%)
83-95
96-112
92-104
Average (%)
90
103
98
1:8
Range (%)
88-100
91-104
88-102
Average (%)
93
98
95
1:16
Range (%)
103-118
93-107
85-98
Average (%)
109
100
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
3.05
9.61
1.07
2.75
10.3
Standard deviation
0.11
0.25
0.86
0.12
0.22
0.88
C V (%)
10.68
8.2
8.95
11.21
8.0
8.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CKM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CKM in samples. No significant cross-reactivity or interference between Mouse CKM and analogues was observed.
