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Mouse Chemokine C-C-Motif Receptor 2 (CCR2) ELISA Kit

SKU:
MODL00228
Product Type:
ELISA Kit
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD192,CC-CKR2,CCR2A,C-C CKR-2,CCR2B,CKR2-A,CKR2-B,CMKBR2,MCP1-R,Monocyte chemoattractant protein 1 receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Chemokine C-C-Motif Receptor 2 (CCR2) ELISA Kit

The Mouse Chemokine C-C Motif Receptor 2 (CCR2) ELISA Kit is a high-quality assay designed for the precise quantification of CCR2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CCR2 is a key chemokine receptor involved in the regulation of immune responses and inflammation. Its role in various diseases, including atherosclerosis, multiple sclerosis, and diabetes, makes it a valuable biomarker for studying the pathophysiology of these conditions and identifying potential therapeutic targets.

With the Mouse CCR2 ELISA Kit, researchers can confidently measure CCR2 levels in mouse samples, advancing their understanding of immune system functions and disease mechanisms. This kit provides a reliable tool for investigating CCR2-related pathways and developing novel treatment strategies.

Product Name:Mouse Chemokine C-C-Motif Receptor 2 (CCR2) ELISA Kit
Product Code:MODL00228
Size:96 Assays
Target:CCR2
Synonyms:CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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