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Mouse Chemokine C-C-Motif Receptor 10 (CCR10) ELISA Kit

SKU:
MODL00227
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JL21
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GPR2,G Protein-Coupled Receptor 2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Chemokine C-C-Motif Receptor 10 (CCR10) ELISA Kit

The Mouse CCR10 (Chemokine C-C motif receptor 10) ELISA Kit is a highly accurate tool for measuring levels of CCR10 in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers precise and reproducible results, making it an indispensable asset for a variety of research endeavors.CCR10 is a key receptor involved in immune cell trafficking and inflammatory responses. Its dysregulation has been associated with various autoimmune diseases, skin disorders, and cancer progression.

By accurately quantifying CCR10 levels, researchers can gain valuable insights into the role of this receptor in disease pathogenesis and potentially identify new therapeutic targets.Overall, the Mouse CCR10 ELISA Kit offers a powerful solution for studying the function and regulation of CCR10 in preclinical research settings, paving the way for innovative discoveries and therapeutic interventions.

Product Name:Mouse Chemokine C-C-Motif Receptor 10 (CCR10) ELISA Kit
Product Code:MODL00227
Size:96 Assays
Target:CCR10
Synonyms:GPR2, G Protein-Coupled Receptor 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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