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Mouse Chemokine C-C-Motif Ligand 1 (CCL1) ELISA Kit

SKU:
MODL00224
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10146
Sensitivity:
32pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
SCYA1,I-309,TCA3,P500,SISe,Small Inducible Cytokine A1,T Lymphocyte Secreted Protein I-309
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Chemokine C-C-Motif Ligand 1 (CCL1) ELISA Kit

The Mouse CCL1 (Chemokine C-C Motif Ligand 1) ELISA Kit is specifically designed for the accurate and sensitive detection of CCL1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high precision and reproducibility, ensuring reliable results for a variety of research applications.CCL1 is an important chemokine in the immune system, playing a role in regulating immune cell migration and activation. It is involved in inflammatory responses and immune cell recruitment to sites of injury or infection.

Measurement of CCL1 levels can provide valuable insights into the immune response in various disease states, making it a valuable tool for research in immunology and inflammation.Overall, the Mouse CCL1 ELISA Kit from AssayGenie is a valuable tool for researchers looking to study the role of CCL1 in immune responses and inflammatory processes in mice, providing accurate and reliable results for a wide range of experimental settings.

Product Name:Mouse Chemokine C-C-Motif Ligand 1 (CCL1) ELISA Kit
Product Code:MODL00224
Size:96 Assays
Target:CCL1
Synonyms:SCYA1, I-309, TCA3, P500, SISe, Small Inducible Cytokine A1, T Lymphocyte Secreted Protein I-309
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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