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Mouse Chaperonin Containing TCP1, Subunit 2 (CCT2) ELISA Kit

SKU:
MODL00230
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P80314
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CCT-beta,CCTB,TCP-1-beta,T-Complex Protein 1 Subunit Beta
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Mouse Chaperonin Containing TCP1, Subunit 2 (CCT2) ELISA Kit

The Mouse Chaperonin Containing TCP1 Subunit 2 (CCT2) ELISA Kit is a powerful tool for the accurate measurement of CCT2 levels in mouse samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.CCT2 is a key component of the chaperonin complex involved in protein folding and assembly.

Its role in maintaining cellular homeostasis and proper protein function makes it a valuable biomarker for studying various diseases and conditions, including neurodegenerative disorders and cancer. By using this ELISA kit, researchers can gain valuable insights into the role of CCT2 in disease progression and potential therapeutic targets.

Product Name:Mouse Chaperonin Containing TCP1, Subunit 2 (CCT2) ELISA Kit
Product Code:MODL00230
Size:96 Assays
Target:CCT2
Synonyms:CCT-beta, CCTB, TCP-1-beta, T-Complex Protein 1 Subunit Beta
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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