Mouse CFL1 (Cofilin 1, Non-Muscle) CLIA Kit (MOES00187)
- SKU:
- MOES00187
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 37.5pg/mL
- Range:
- 62.5-4000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- N-Cofilin
- Reactivity:
- Mouse
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | Mouse CFL1 (Cofilin 1, Non-Muscle) CLIA Kit |
SKU: | MOES00187 |
Target: | Mouse CFL1 (Cofilin 1, Non-Muscle) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 37.50 pg/mL |
Detection range: | 62.50-4000 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CFL1. You can calculate the concentration of Mouse CFL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Tissue homogenates; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Mouse CFL1 concentrations in Tissue homogenates. |
Specificity: | This kit recognizes Mouse CFL1 in samples. No significant cross-reactivity or interference between Mouse CFL1 and analogues was observed. |