The Mouse CFH (Complement Factor H) ELISA Kit is a specialized assay designed for the precise and quantitative detection of Complement Factor H levels in mouse biological samples. Complement Factor H is a key regulator of the innate immune response, playing a critical role in the modulation of the complement system, host defense, and inflammation. Our ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for researchers studying the intricate mechanisms of the immune system and inflammatory responses.
With stringent quality control measures in place, this kit delivers robust performance, making it an ideal choice for investigations focused on the functions and implications of Complement Factor H in various physiological and pathological conditions.
Product Name:
Mouse CFH (Complement Factor H) ELISA Kit
SKU:
AEES00216
Target:
Mouse CFH (Complement Factor H)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CFH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CFH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CFH. You can calculate the concentration of Mouse CFH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-99
89-100
88-101
Average (%)
94
96
93
1:4
Range (%)
100-111
98-106
95-104
Average (%)
105
103
99
1:8
Range (%)
89-97
95-105
88-95
Average (%)
92
99
92
1:16
Range (%)
91-102
94-103
95-105
Average (%)
95
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
95-107
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.12
23.79
81.59
8.56
23.63
90.21
Standard deviation
0.49
1.18
4.41
0.48
1.12
4.15
C V (%)
5.37
4.96
5.41
5.61
4.74
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CFH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CFH in samples. No significant cross-reactivity or interference between Mouse CFH and analogues was observed.
