Mouse CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit (MOES00179)
- SKU:
- MOES00179
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 18.75pg/mL
- Range:
- 31.25-2000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- TNFSF5, CD40LG, CD154, HIGM1, IGM, IMD3, T-BAM, TRAP, Gp39
- Reactivity:
- Mouse
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | Mouse CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit |
SKU: | MOES00179 |
Target: | Mouse CD40L (Cluster of Differentiation 40 Ligand) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 18.75 pg/mL |
Detection range: | 31.25-2000 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD40L. You can calculate the concentration of Mouse CD40L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Serum, plasma and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Mouse CD40L concentrations in Serum, plasma and other biological fluids. |
Specificity: | This kit recognizes Mouse CD40L in samples. No significant cross-reactivity or interference between Mouse CD40L and analogues was observed. |