The Mouse CD3-Epsilon Monoclonal Antibody (APC Conjugated) is a powerful tool for researchers studying T-cell biology and immunology. This antibody, clone 145-2C11, is specifically designed to detect the CD3-epsilon antigen, a key component of the T-cell receptor complex.Ideal for flow cytometry applications, the Mouse CD3-Epsilon Monoclonal Antibody (APC Conjugated) offers high sensitivity and specificity in identifying and analyzing T cells in various samples. Its conjugation with allophycocyanin (APC) allows for easy and efficient detection using fluorescent microscopy or flow cytometry techniques.
The CD3-epsilon antigen is vital for T-cell activation and function, making this antibody indispensable for investigating T-cell responses in immune system studies, infectious diseases, and cancer research. With its reliable performance and accurate detection capabilities, the Mouse CD3-Epsilon Monoclonal Antibody (APC Conjugated) is a valuable asset for scientists exploring the intricacies of T-cell biology and immune responses.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P22646
Gene ID:
12501
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.