The Mouse CD274 (PD-L1) Monoclonal Antibody (APC Conjugated) 10F.9G2 (AGEL1643) is a powerful tool for researchers studying the immune system and its interactions with cancer cells. This monoclonal antibody targets CD274, also known as programmed death-ligand 1 (PD-L1), a key immune checkpoint protein that plays a critical role in regulating immune responses.PD-L1 is a major checkpoint protein that can inhibit T cell activation and contribute to immune evasion by cancer cells. By targeting PD-L1 with this specific antibody, researchers can effectively detect and analyze the expression of PD-L1 in various cell types, providing valuable insights into the immune evasion mechanisms employed by cancer cells.The APC conjugation of this monoclonal antibody facilitates its use in flow cytometry and immunofluorescence applications, allowing for the precise detection and quantification of PD-L1 expression on the cell surface.
This antibody has been rigorously validated for use in research settings, ensuring reliable and reproducible results.Researchers investigating the role of PD-L1 in cancer progression, immune evasion, and immunotherapy will benefit greatly from the specificity and sensitivity of the Mouse CD274 (PD-L1) Monoclonal Antibody (APC Conjugated) 10F.9G2 (AGEL1643). By understanding the expression patterns and regulation of PD-L1, new insights can be gained into designing novel therapeutic strategies to enhance anti-tumor immune responses in cancer patients.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
Q9EP73
Gene ID:
60533
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.
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