The Mouse CD25 Monoclonal Antibody PE-Cyanine7 Conjugated (PC 61.5.3) is a valuable tool for research involving CD25, a cell surface protein associated with T-cell activation and regulatory T-cell function. This antibody, specifically designed for use in flow cytometry applications, is raised against mouse samples and provides high reactivity and specificity.CD25, also known as IL-2 receptor alpha chain, is crucial for the activation and proliferation of T cells, playing a key role in immune responses and tolerance. By targeting CD25, researchers can investigate the dynamics of T-cell activation and regulatory T-cell populations, offering insights into immune regulation in various disease settings.
The Mouse CD25 Monoclonal Antibody PE-Cyanine7 Conjugated (PC 61.5.3) facilitates the identification and characterization of CD25-expressing cells in experimental models, aiding in the understanding of immune responses, tolerance mechanisms, and therapeutic interventions. Its application in flow cytometry ensures precise and reliable detection of CD25, making it an indispensable tool for immunology studies and research on autoimmune diseases, cancer immunotherapy, and transplantation.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.