The Mouse CD25 Monoclonal Antibody PE Conjugated (PC61.5.3) is a powerful tool for research involving CD25, a key cell surface molecule involved in immune regulation and T cell activation. This monoclonal antibody, conjugated with phycoerythrin (PE), is specifically designed for flow cytometry applications, enabling precise detection and analysis of CD25 expression in mouse samples.CD25, also known as interleukin-2 receptor alpha chain, plays a crucial role in T cell activation and immune response regulation. Its expression is indicative of T cell activation and differentiation, making it a valuable marker for studying immune responses in various experimental settings.
The PE-conjugated PC61.5.3 antibody allows for single-step staining and flow cytometry analysis, providing researchers with a highly sensitive and specific tool for investigating CD25 expression in different cell populations.With its ability to selectively bind to CD25-expressing cells, this monoclonal antibody is an essential resource for immunologists, cancer researchers, and scientists studying autoimmune diseases. By facilitating the identification and quantification of CD25-positive cells, this antibody contributes to a deeper understanding of immune function and holds promise for advancing research in areas like immunotherapy, autoimmunity, and transplantation.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.