The Mouse CD25 Monoclonal Antibody (Biotin Conjugated) - PC61.5.3 (AGEL0372) is a highly specific antibody designed for researchers studying CD25, a cell surface molecule crucial for immune function. This monoclonal antibody, originating from mouse cells, is conjugated with biotin for enhanced detection capabilities in various experimental techniques.CD25, also known as the interleukin-2 receptor alpha chain, plays a vital role in T cell activation and regulatory T cell function. The antibody binds specifically to the CD25 protein, allowing for precise detection and analysis in different cell types and biological samples.
Its conjugation with biotin further expands its utility in applications such as flow cytometry, immunohistochemistry, and immunoprecipitation.Researchers investigating immune responses, T cell biology, and immune-related disorders will find the Mouse CD25 Monoclonal Antibody (Biotin Conjugated) - PC61.5.3 (AGEL0372) to be a valuable tool in their studies. By targeting CD25, this antibody provides insights into T cell activation mechanisms, immunoregulation, and potential therapeutic targets for immune-related diseases.
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P01590
Gene ID:
16184
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.