Purified Anti-Mouse CD22 Antibody [Cy34.1] (AGEL0123)
- SKU:
- AGEL0123
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Mouse
- Host Species:
- Mouse
- Isotype:
- IgG1
- Clone:
- Cy34.1
- Conjugation:
- Unconjugated
- Applications:
- FC
Frequently bought together:
Description
Product Title: | Purified Anti-Mouse CD22 Antibody [Cy34.1] |
SKU: | AGEL0123 |
Size: | 25µg, 100µg |
Clone No: | Cy34.1 |
Synonyms: | B-cell receptor CD22;Cd22;B-lymphocyte cell adhesion molecule;BL-CAM;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14;CD22;Lyb-8; Siglec2; |
Host Species: | Mouse |
Reactivity: | Mouse |
Application: | FCM |
Isotype: | Mouse IgG1, κ |
Isotype Control: | Purified Mouse IgG1, κ Isotype Control[MOPC-21] |
Form: | Liquid |
Conjugation: | Unconjugated |
Recommended Use: | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Swissprot: | P35329 |
Gene ID: | 12483 |
Storage Buffer: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Stability & Storage: | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Background: | The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb. |