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PE/Cyanine5 Anti-Mouse CD162 Antibody [4RA10] (AGEL0842)

SKU:
AGEL0842
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
€389 - €889
Frequently bought together:

Description

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PE/Cyanine5 Anti-Mouse CD162 Antibody [4RA10]

The Mouse CD162 Monoclonal Antibody PE-Cyanine5 Conjugated (4RA10) is an essential tool for research involving the CD162 protein, a cell surface molecule known for its role in mediating cell adhesion and migration processes. This antibody, developed for mouse samples, is conjugated with PE-Cyanine5 for enhanced detection in flow cytometry and fluorescence microscopy applications.CD162, also called P-selectin glycoprotein ligand-1 (PSGL-1), is a key player in immune cell recruitment to sites of inflammation and tissue damage. By binding to selectins expressed on endothelial cells, CD162 facilitates the tethering and rolling of leukocytes, ultimately promoting their adhesion and transmigration.

This critical function makes CD162 a central focus in studies related to immune responses, inflammatory disorders, and vascular biology.Researchers investigating cell trafficking, leukocyte-endothelial interactions, and inflammatory pathways can benefit from the specificity and sensitivity of the Mouse CD162 Monoclonal Antibody PE-Cyanine5 Conjugated (4RA10). Its ability to target the CD162 protein with high affinity enables precise detection and analysis in various experimental models, paving the way for insights into the mechanisms underlying immune cell behavior and inflammatory processes.

Product Title:PE/Cyanine5 Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0842
Size:50 Tests, 100 Tests, 200 Tests
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:PE/Cyanine5 Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:PE/Cyanine 5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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