The Mouse CD14 Monoclonal Antibody (PERCP-Cyanine5.5 Conjugated) is a powerful tool for researchers studying CD14, a well-known cell surface marker predominantly found on monocytes and macrophages. This monoclonal antibody, named SA14-2 (AGEL1878), is specifically designed for flow cytometry applications, offering high specificity and sensitivity in detecting CD14 expression in mouse samples.CD14 plays a crucial role in the recognition of bacterial lipopolysaccharides (LPS) and initiating immune responses. By targeting CD14 with this monoclonal antibody, researchers can effectively analyze and quantify CD14-positive cells in various experimental settings, including immunology and infectious disease studies.
The PERCP-Cyanine5.5 conjugation enhances the fluorescence signal, enabling precise identification and sorting of CD14-expressing cells with flow cytometry techniques.With its ability to target CD14 and facilitate in-depth cellular analysis, the Mouse CD14 Monoclonal Antibody (PERCP-Cyanine5.5 Conjugated) is an essential tool for understanding immune responses, inflammation, and host-pathogen interactions in mouse models. Researchers can leverage this antibody to advance knowledge in immunology, microbiology, and related fields, ultimately contributing to the development of targeted therapies and treatment strategies for immune-related disorders and infections.
CD 14;Myeloid cell-specific leucine-rich glycoprotein;Monocyte differentiation antigen CD14;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG2a, κ
Isotype Control:
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3]
Form:
Liquid
Conjugation:
PerCP/Cyanine 5.5
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P10810
Gene ID:
12475
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.