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Mouse Cathepsin Z (CTSZ) ELISA Kit

SKU:
MODL00330
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WUU7
Sensitivity:
7.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CTS-Z,CTSX,Cathepsin P,Cathepsin X
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Cathepsin Z (CTSZ) ELISA Kit

The Mouse Cathepsin Z (CTSZ) ELISA Kit is specifically designed for the quantitative detection of Cathepsin Z levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and precision, ensuring accurate and reproducible results for a variety of research applications.Cathepsin Z is a protease enzyme that plays a crucial role in the regulation of various biological processes, including immune response and tissue remodeling. Elevated levels of Cathepsin Z have been linked to inflammatory diseases, cancer progression, and tissue degeneration, highlighting its significance as a potential biomarker for studying these conditions and developing targeted therapies.

With its reliable performance and ease of use, the Mouse Cathepsin Z (CTSZ) ELISA Kit is an essential tool for researchers investigating the role of Cathepsin Z in disease pathogenesis and exploring therapeutic interventions.

Product Name:Mouse Cathepsin Z (CTSZ) ELISA Kit
Product Code:MODL00330
Size:96 Assays
Target:CTSZ
Synonyms:CTS-Z, CTSX, Cathepsin P, Cathepsin X
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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