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Mouse Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

SKU:
MODL00198
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51437
Sensitivity:
0.129ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CAP18,FALL39,HSD26,LL37,18 kDa Cationic Antimicrobial Protein,Antibacterial protein FALL-39,FALL-39 peptide antibiotic
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit

The Mouse Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit is specifically designed for the accurate measurement of CAMP levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CAMP is an important antimicrobial peptide that plays a key role in the innate immune response, protecting against bacterial infections and promoting wound healing.

The levels of CAMP in the body can provide valuable insights into the immune system function and response to infections, making it a valuable biomarker for studying immune-related conditions and developing potential therapeutic interventions.Overall, the Mouse CAMP ELISA Kit is a reliable and effective tool for researchers looking to investigate the role of CAMP in immune function and disease processes in mouse models.

Product Name:Mouse Cathelicidin Antimicrobial Peptide (CAMP) ELISA Kit
Product Code:MODL00198
Size:96 Assays
Target:CAMP
Synonyms:CAP18, FALL39, HSD26, LL37, 18 kDa Cationic Antimicrobial Protein, Antibacterial protein FALL-39, FALL-39 peptide antibiotic
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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