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Mouse Caspase 9 (CASP9) ELISA Kit

SKU:
MODL00210
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8C3Q9
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
APAF3,ICE-LAP6,MCH6,Apoptosis-Related Cysteine Peptidase,Cysteinyl Aspartate Specific Proteinases 9,Apoptotic protease-activating factor 3,ICE-like apoptotic protease 6
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Caspase 9 (CASP9) ELISA Kit

The Mouse Caspase-9 (CASP9) ELISA Kit is a highly sensitive and specific tool designed for the accurate measurement of caspase-9 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Caspase-9 is a key protein in the apoptosis pathway, playing a critical role in the regulation of cell death. Dysregulation of this protein has been implicated in various diseases, including cancer and neurodegenerative disorders.

Therefore, the measurement of caspase-9 levels using this ELISA kit can provide valuable insights into these conditions and aid in the development of potential therapies.Overall, the Mouse Caspase-9 ELISA Kit is a powerful tool for researchers looking to study apoptosis and its implications in disease pathogenesis, offering high-quality performance and accuracy for their experiments.

Product Name:Mouse Caspase 9 (CASP9) ELISA Kit
Product Code:MODL00210
Size:96 Assays
Target:CASP9
Synonyms:APAF3, ICE-LAP6, MCH6, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 9, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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