null

Mouse Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit

SKU:
MODL00302
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P15089
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MC-CPA,Mast cell carboxypeptidase A
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit

The Mouse Carboxypeptidase A3 (Mast Cell CPA3) ELISA Kit is specifically designed for the accurate measurement of mast cell carboxypeptidase A3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Mast cell carboxypeptidase A3 is an important enzyme involved in the regulation of inflammatory responses and is primarily found in mast cells.

Its dysregulation has been associated with various inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With the Mouse Carboxypeptidase A3 (Mast Cell CPA3) ELISA Kit, researchers can gain valuable insights into the role of this enzyme in inflammatory processes and develop new strategies for targeted therapeutic interventions.

Product Name:Mouse Carboxypeptidase A3, Mast Cell (CPA3) ELISA Kit
Product Code:MODL00302
Size:96 Assays
Target:CPA3
Synonyms:MC-CPA, Mast cell carboxypeptidase A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products