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Mouse Carbohydrate Antigen 125 (CA125) ELISA Kit

SKU:
MODL00189
Product Type:
ELISA Kit
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MUC16,Cancer Antigen 125,Mucin 16,Cell Surface Associated,Ovarian Cancer-Related Tumor Marker CA125,Ovarian Carcinoma Antigen CA125
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Carbohydrate Antigen 125 (CA125) ELISA Kit

The Mouse CA125 (Carbohydrate Antigen 125) ELISA Kit is a powerful tool for the precise detection of CA125 levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring consistent and accurate results that are essential for various research applications.CA125, also known as Mucin-16, is a protein that is commonly used as a tumor marker for ovarian cancer. Elevated levels of CA125 in the body have been linked to various cancers and inflammatory conditions, making it a valuable biomarker for understanding disease progression and development.

The Mouse CA125 ELISA Kit offers researchers a reliable method for studying the role of CA125 in cancer and other related diseases, providing valuable insights into potential diagnostic and therapeutic strategies. Trust in this kit for robust and reproducible results that will advance your research efforts significantly.

Product Name:Mouse Carbohydrate Antigen 125 (CA125) ELISA Kit
Product Code:MODL00189
Size:96 Assays
Target:CA125
Synonyms:MUC16, Cancer Antigen 125, Mucin 16,Cell Surface Associated, Ovarian Cancer-Related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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