The Mouse Calu (Calumenin) CLIA Kit is specifically designed for the precise measurement of Calumenin levels in mouse serum, plasma, and tissue samples. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for various research purposes.Calumenin is a vital protein involved in calcium homeostasis and regulation of cell proliferation. It plays a crucial role in various physiological processes and has been implicated in conditions such as cancer, diabetes, and neurodegenerative diseases.
Therefore, studying Calumenin levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Mouse Calu (Calumenin) CLIA Kit is an essential tool for researchers investigating the role of Calumenin in health and disease, offering reliable data for advancing scientific understanding and potentially identifying new treatment options. Visit the provided link for more information and to purchase the kit.
Product Name:
Mouse CALU (Calumenin) CLIA Kit
SKU:
MOES00140
Target:
Mouse CALU (Calumenin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CALU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CALU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CALU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CALU. You can calculate the concentration of Mouse CALU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
103-119
98-111
Average (%)
102
109
105
1:4
Range (%)
95-112
94-110
97-111
Average (%)
102
102
104
1:8
Range (%)
85-96
103-116
96-112
Average (%)
91
109
102
1:16
Range (%)
88-98
88-103
92-110
Average (%)
93
94
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.08
23.53
90.47
10.9
25.61
98.24
Standard deviation
1.06
2.06
6.73
1.19
1.81
8.66
C V (%)
10.52
8.75
7.44
10.92
7.07
8.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CALU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CALU in samples. No significant cross-reactivity or interference between Mouse CALU and analogues was observed.
