null

Mouse Calpain 1, Large Subunit (CAPN1) ELISA Kit

SKU:
MODL00200
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O35350
Sensitivity:
0.58ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
PIG30,CANPL1,MuCANP,MuCL,Calpain-1 Catalytic Subunit,Calcium-Dependent Protease 1,Calcium-activated neutral proteinase 1,Calpain mu-type,Micromolar-calpain
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Calpain 1, Large Subunit (CAPN1) ELISA Kit

The Mouse Calpain-1 Large Subunit (CAPN1) ELISA Kit is specifically designed for the accurate quantification of CAPN1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CAPN1 is a key enzyme involved in regulating various cellular processes, including cell migration, proliferation, and apoptosis. Dysregulation of CAPN1 has been implicated in various diseases such as neurodegenerative disorders, muscular dystrophy, and cancer.

Therefore, measuring CAPN1 levels can provide valuable insights into disease progression and potential therapeutic interventions.With easy-to-follow instructions and quick assay procedures, the Mouse CAPN1 ELISA Kit is a reliable tool for researchers studying the role of calpain-1 in disease pathology and exploring novel treatment strategies.

Product Name:Mouse Calpain 1, Large Subunit (CAPN1) ELISA Kit
Product Code:MODL00200
Size:96 Assays
Target:CAPN1
Synonyms:PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products