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Mouse Calcitonin (CT) ELISA Kit

SKU:
MODL00319
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70160
Sensitivity:
4.45pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Competitive
Synonyms:
CALCA,CALC1,CGRP-I,CGRP1,KC,CCP,PDN-21,Katacalcin,Calcitonin-Related Polypeptide Alpha,Calcitonin carboxyl-terminal peptide
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Calcitonin (CT) ELISA Kit

The Mouse Calcitonin (CT) ELISA Kit is specifically designed for the precise measurement of calcitonin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable tool for various research applications.Calcitonin is a key hormone that regulates calcium levels in the body and is primarily produced by the thyroid gland. It plays a crucial role in bone metabolism, particularly in inhibiting bone resorption.

Dysregulation of calcitonin levels has been linked to conditions such as osteoporosis, hypercalcemia, and certain types of cancer, highlighting its importance as a biomarker in studying and developing treatments for these diseases.Get your Mouse Calcitonin (CT) ELISA Kit today and delve into the world of calcitonin research with confidence and precision.

Product Name:Mouse Calcitonin (CT) ELISA Kit
Product Code:MODL00319
Size:96 Assays
Target:CT
Synonyms:CALCA, CALC1, CGRP-I, CGRP1, KC, CCP, PDN-21, Katacalcin, Calcitonin-Related Polypeptide Alpha, Calcitonin carboxyl-terminal peptide
Detection Method:Sandwich
Range:15.6-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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