The Mouse Cadherin-5 (CDH5) ELISA Kit is specifically designed for the precise detection of Cadherin-5 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.Cadherin-5, also known as vascular endothelial cadherin, is a critical protein involved in vascular development and endothelial cell function. It plays a key role in maintaining vascular integrity and regulating endothelial cell behavior. Dysregulation of Cadherin-5 has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its importance as a potential biomarker for disease progression and therapeutic development.
Overall, the Mouse Cadherin-5 (CDH5) ELISA Kit is a reliable and efficient solution for researchers studying vascular biology, angiogenesis, and endothelial cell function in mouse models. Its accuracy and precision make it an essential tool for advancing our understanding of Cadherin-5 biology and its implications in various pathological conditions.
Product Name:
Mouse Cadherin 5 (CDH5) ELISA Kit
Product Code:
MODL00243
Size:
96 Assays
Target:
CDH5
Synonyms:
CD144, 7B4, Cadherin 5 Type 2, VE-Cadherin, Cadherin,Vascular Endothelial
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
