The Mouse C5a (Complement Component 5a) ELISA Kit is specifically designed for the accurate measurement of C5a levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are essential for a variety of research applications.C5a is a key component of the complement system that plays a critical role in inflammation and immune response. It is involved in various physiological and pathological processes, including host defense, autoimmunity, and inflammatory diseases.
Monitoring C5a levels can provide valuable insights into the immune system and help in the development of targeted therapies for inflammatory disorders.With its easy-to-use protocol and robust performance, the Mouse C5a ELISA Kit from Assay Genie is an indispensable tool for researchers studying the role of complement system activation in health and disease. Order yours today to advance your research efforts in immunology and inflammation.
Product Name:
Mouse C5a (Complement Component 5a) ELISA Kit
SKU:
MOES00902
Target:
Mouse C5a (Complement Component 5a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C5a. You can calculate the concentration of Mouse C5a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
83-99
91-105
Average (%)
92
90
97
1:4
Range (%)
92-104
81-94
85-99
Average (%)
97
86
90
1:8
Range (%)
87-103
85-98
86-101
Average (%)
94
92
93
1:16
Range (%)
86-100
87-101
87-98
Average (%)
92
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
89-106
97
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.42
416.47
1834.37
181.1
424.57
1744.6
Standard deviation
10
19.2
76.68
12.68
25.13
58.27
C V (%)
5.25
4.61
4.18
7.0
5.92
3.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C5a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C5a in samples. No significant cross-reactivity or interference between Mouse C5a and analogues was observed.
