The Mouse C5 Complement Component 5 ELISA Kit is specifically designed for the precise measurement of C5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.C5 is a key component of the complement system involved in immune responses and inflammation. It plays a critical role in defense against pathogens and maintaining immune homeostasis.
Abnormalities in C5 levels have been associated with various diseases, including autoimmune disorders and infectious diseases, highlighting its significance as a biomarker for disease progression and therapeutic development.By using the Mouse C5 Complement Component 5 ELISA Kit, researchers can accurately quantify C5 levels, advancing their understanding of immune responses and potentially identifying new targets for therapeutic interventions.
Product Name:
Mouse C5 (Complement Component 5) ELISA Kit
SKU:
MOES00896
Target:
Mouse C5 (Complement Component 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C5. You can calculate the concentration of Mouse C5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
87-101
97-111
Average (%)
101
93
103
1:4
Range (%)
89-101
81-95
87-101
Average (%)
96
88
93
1:8
Range (%)
87-100
88-100
85-95
Average (%)
93
93
90
1:16
Range (%)
94-109
82-92
81-95
Average (%)
99
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.19
3.99
0.45
1.07
3.63
Standard deviation
0.02
0.06
0.2
0.02
0.06
0.15
C V (%)
4.26
5.04
5.01
4.44
5.61
4.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse C5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse C5 in samples. No significant cross-reactivity or interference between Mouse C5 and analogues was observed.
