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Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

SKU:
MODL01134
Product Type:
ELISA Kit
Sensitivity:
33pg/mL
Range:
78.1-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
MCF3,ROS,c-Ros-1,v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1,Proto-oncogene tyrosine-protein kinase ROS,Receptor tyrosine kinase c-ros oncogene 1
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

The Mouse c-ros Oncogene 1 receptor tyrosine kinase (ROS1) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of ROS1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it well-suited for a variety of research applications.ROS1 is a receptor tyrosine kinase that plays a critical role in cell growth, survival, and differentiation.

Dysregulation of ROS1 has been implicated in various cancers, making it an important target for cancer research and potential therapies. By accurately measuring ROS1 levels, researchers can gain valuable insights into the role of this protein in tumorigenesis and potentially identify new treatment strategies.

Product Name:Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit
Product Code:MODL01134
Size:96 Assays
Target:ROS1
Synonyms:MCF3, ROS, c-Ros-1, v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1, Proto-oncogene tyrosine-protein kinase ROS, Receptor tyrosine kinase c-ros oncogene 1
Detection Method:Sandwich
Range:78.1-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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