Mouse Bromodomain-Containing Protein 4 (BRD4) ELISA Kit
The Mouse BRD4 (Bromodomain-containing protein 4) ELISA Kit is a powerful tool for the precise measurement of BRD4 levels in mouse samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable resource for a variety of research studies.BRD4 is a key protein involved in epigenetic regulation and transcriptional activation, playing a critical role in various cellular processes such as gene expression and cell cycle progression. Dysregulation of BRD4 has been implicated in numerous diseases, including cancer and inflammatory disorders, highlighting its potential as a therapeutic target and diagnostic marker.
By utilizing the Mouse BRD4 ELISA Kit, researchers can gain valuable insights into the function and expression levels of BRD4 in mouse models, facilitating advancements in drug development, disease research, and personalized medicine. Trust in the reliability and precision of this kit to drive your research forward and uncover new possibilities in the field of molecular biology.
Product Name:
Mouse Bromodomain-Containing Protein 4 (BRD4) ELISA Kit
Product Code:
MODL00171
Size:
96 Assays
Target:
BRD4
Synonyms:
Mitotic chromosome-associated protein(MCAP)
Detection Method:
Sandwich
Range:
31.2-2000pg/ml
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
