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Mouse Brain Natriuretic Peptide (BNP) ELISA Kit

SKU:
MODL00167
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P40753
Sensitivity:
12.42pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
GC-B,B-Type Natriuretic Peptide,Ventricular Natriuretic Peptide,Gamma-brain natriuretic peptide,Brain natriuretic peptide 32
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Mouse Brain Natriuretic Peptide (BNP) ELISA Kit

The Mouse Brain Natriuretic Peptide (BNP) ELISA Kit is specifically designed for the accurate detection of BNP levels in mouse brain tissue samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results that are ideal for a variety of research applications.BNP is a hormone secreted by the heart in response to increased pressure and volume overload, making it a key biomarker for heart failure and other cardiovascular conditions.

By measuring BNP levels in mouse brain tissue, researchers can gain valuable insights into the role of this hormone in neurological conditions and potential therapeutic interventions.Overall, the Mouse Brain Natriuretic Peptide (BNP) ELISA Kit provides researchers with a reliable tool for studying the intricate relationship between BNP and brain function, offering valuable data for advancing our understanding of cardiovascular and neurological disorders.

Product Name:Mouse Brain Natriuretic Peptide (BNP) ELISA Kit
Product Code:MODL00167
Size:96 Assays
Target:BNP
Synonyms:GC-B, NPPB, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-brain natriuretic peptide, Brain natriuretic peptide 32
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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