The Mouse Bone Sialoprotein (BSP) ELISA Kit is a professional tool for the precise measurement of BSP levels in mouse serum, plasma, and tissue culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it a valuable asset for various research purposes.Bone sialoprotein is a critical component in bone tissue, contributing to bone mineralization and remodeling processes. It is involved in cell adhesion, differentiation, and signaling, playing a crucial role in bone development and homeostasis.
By measuring BSP levels, researchers can gain insights into bone metabolism, skeletal disorders, and potential therapeutic interventions.Overall, the Mouse BSP ELISA Kit provides researchers with a reliable method for studying bone biology, investigating skeletal diseases, and exploring potential treatments for bone-related conditions. Get your kit today and advance your research in the field of bone sialoprotein biology.
Product Name:
Mouse Bone Sialoprotein (BSP) ELISA Kit
Product Code:
MODL00172
Size:
96 Assays
Target:
BSP
Synonyms:
IBSP, Bsp, BspII, SpII, Integrin-Binding Bone Sialoprotein, Cell-binding sialoprotein, Bone sialoprotein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
