The Mouse Big Dynorphin (Dyn) ELISA Kit is specifically designed for the quantitative measurement of Big Dynorphin levels in mouse serum, plasma, and tissue lysates. This kit offers high precision and accuracy, allowing for consistent and reliable results for various research applications.Big Dynorphin, a neuropeptide derived from the precursor protein prodynorphin, has been identified as an important regulator of pain modulation, mood regulation, and stress responses in the central nervous system.
Dysregulation of Big Dynorphin has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its user-friendly protocol and quick assay turnaround time, the Mouse Big Dynorphin (Dyn) ELISA Kit is a valuable tool for researchers looking to investigate the role of Big Dynorphin in physiological and pathological processes in mouse models.
Product Name:
Mouse Big Dynorphin (Dyn) ELISA Kit
Product Code:
MODL00396
Size:
96 Assays
Target:
Dyn
Synonyms:
B-DYN, Proenkephalin-B, Dyn-A17
Detection Method:
Competition
Range:
0.312-20mIU/L
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
91-99
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
