The Mouse ATXN1 (Ataxin-1) CLIA Kit is a highly reliable and specific assay designed for the accurate detection of ATXN1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.ATXN1 is a critical protein involved in the development of neurodegenerative disorders, particularly spinocerebellar ataxias. It plays a key role in regulating cell function and neuronal survival, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.
With its advanced technology and high-quality components, the Mouse ATXN1 CLIA Kit is an essential tool for researchers looking to investigate the role of ATXN1 in neurodegenerative diseases and advance our understanding of these complex conditions. Order yours today to enhance your research and uncover valuable insights into neurological disorders.
Product Name:
Mouse ATXN1 (Ataxin 1) CLIA Kit
SKU:
MOES00105
Target:
Mouse ATXN1 (Ataxin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ATXN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ATXN1. You can calculate the concentration of Mouse ATXN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
95-108
93-107
Average (%)
96
102
101
1:4
Range (%)
87-104
99-111
96-112
Average (%)
95
105
103
1:8
Range (%)
90-106
99-116
103-119
Average (%)
98
106
109
1:16
Range (%)
86-97
84-96
91-106
Average (%)
91
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.51
66.19
171.34
24.48
67.43
174.84
Standard deviation
2.38
6.0
11.7
2.45
7.41
12.05
C V (%)
9.71
9.06
6.83
10.01
10.99
6.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ATXN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ATXN1 in samples. No significant cross-reactivity or interference between Mouse ATXN1 and analogues was observed.
