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Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit

SKU:
MODL00132
Product Type:
ELISA Kit
Sensitivity:
27.8pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
X,K-ATPase subunit beta-m,X/potassium-transporting ATPase subunit beta-m
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

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Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit

The Mouse ATPase Na/K Transporting Beta 4 Polypeptide (ATP1B4) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ATP1B4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a wide range of research applications.ATP1B4 is a critical protein involved in ion transport and maintaining cellular membrane potential, making it essential for proper cell function.

Dysregulation of ATP1B4 has been linked to various diseases and conditions, including neurological disorders and cardiovascular diseases. Therefore, this kit serves as a valuable tool for studying ATP1B4 and its role in various physiological processes and diseases, ultimately aiding in the development of potential therapies.

Product Name:Mouse ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit
Product Code:MODL00132
Size:96 Assays
Target:ATP1b4
Synonyms:X,K-ATPase subunit beta-m, X/potassium-transporting ATPase subunit beta-m
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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