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Mouse ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit

SKU:
MODL00129
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6PIE5
Sensitivity:
0.042ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FHM2,MHP2,Migraine,Hemiplegic 2,Sodium pump subunit alpha-2,Sodium/potassium-transporting ATPase subunit alpha-2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit

The Mouse ATPase Na+/K+ Transporting Alpha 2 Polypeptide (ATP1A2) ELISA Kit is specifically designed for the accurate measurement of ATP1A2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ATP1A2 is a key enzyme involved in ion transport, specifically in maintaining the balance of sodium and potassium ions across cell membranes. Dysfunction of ATP1A2 has been implicated in various diseases and conditions, including neurological disorders, cardiovascular diseases, and kidney disorders.

Studying ATP1A2 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its reliable performance and versatility, the Mouse ATPase Na+/K+ Transporting Alpha 2 Polypeptide (ATP1A2) ELISA Kit is an essential tool for researchers investigating the role of ATP1A2 in health and disease.

Product Name:Mouse ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit
Product Code:MODL00129
Size:96 Assays
Target:ATP1a2
Synonyms:FHM2, MHP2, Migraine,Hemiplegic 2, Sodium pump subunit alpha-2, Sodium/potassium-transporting ATPase subunit alpha-2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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