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Mouse ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit

SKU:
MODL00128
Product Type:
ELISA Kit
Sensitivity:
0.042ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Sodium pump subunit alpha-1,Na(+)/K(+) ATPase alpha-1 subunit,Sodium/potassium-transporting ATPase subunit alpha-1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit

The Mouse ATPase Na+/K+ Transporting Alpha 1 Polypeptide (ATP1A1) ELISA Kit is specifically designed for the accurate and sensitive detection of ATP1A1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high specificity and precision, ensuring reliable and reproducible results for a variety of research applications.ATP1A1 is a key enzyme responsible for the transportation of sodium and potassium ions across cell membranes, playing a crucial role in maintaining proper cell function and ion balance.

Dysregulation of ATP1A1 has been implicated in various diseases such as hypertension, heart failure, and kidney disorders, making it an important target for studying disease mechanisms and potential therapeutic interventions.Overall, the Mouse ATP1A1 ELISA Kit provides researchers with a valuable tool for investigating the role of ATP1A1 in physiological and pathological processes, ultimately advancing our understanding of ion transport and its impact on health and disease.

Product Name:Mouse ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit
Product Code:MODL00128
Size:96 Assays
Target:ATP1a1
Synonyms:Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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