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Mouse ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit

SKU:
MODL00134
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9R0K7
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PMCA2,Plasma Membrane Ca2+ Pump 2,Plasma Membrane Calcium-Transporting ATPase 2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit

The Mouse ATPase Ca++ transporting plasma membrane 2 (ATP2B2) ELISA Kit is a high-quality assay designed for the accurate measurement of ATP2B2 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ATP2B2 is an important protein involved in calcium ion transport across cell membranes, playing a vital role in maintaining cellular calcium balance.

Dysregulation of ATP2B2 has been implicated in various diseases including cardiovascular disorders and neurodegenerative conditions. Therefore, studying ATP2B2 levels can provide valuable insights into these diseases and potential therapeutic targets.Overall, the Mouse ATP2B2 ELISA Kit is a valuable tool for researchers looking to investigate the role of ATP2B2 in physiological and pathological processes, offering precise and accurate measurements for in-depth analysis.

Product Name:Mouse ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
Product Code:MODL00134
Size:96 Assays
Target:ATP2B2
Synonyms:PMCA2, Plasma Membrane Ca2+ Pump 2, Plasma Membrane Calcium-Transporting ATPase 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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