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Mouse ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

SKU:
MODL00133
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O55143
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ATP2B,DAR,SERCA2,Calcium pump 2,Endoplasmic reticulum class 1/2 Ca(2+) ATPase,Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

The Mouse ATPase Ca++ transporting cardiac muscle slow twitch 2 (ATP2A2) ELISA Kit is a cutting-edge assay designed for the precise quantification of ATP2A2 levels in mouse samples such as serum, plasma, and tissue lysates. This high-quality kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.ATP2A2 is a key enzyme involved in the regulation of calcium transport in cardiac muscle cells, essential for maintaining proper heart function.

Dysregulation of ATP2A2 has been linked to cardiac disorders and heart failure, highlighting its significance as a potential target for therapy development and biomarker identification.With its advanced technology and reliability, the Mouse ATPase Ca++ transporting cardiac muscle slow twitch 2 (ATP2A2) ELISA Kit is an invaluable tool for researchers studying cardiovascular diseases and exploring novel treatment strategies.

Product Name:Mouse ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit
Product Code:MODL00133
Size:96 Assays
Target:ATP2A2
Synonyms:ATP2B, DAR, SERCA2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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