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Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit

SKU:
MODL00139
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WU02
Sensitivity:
0.071ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
AVPR1-B,AVPR3,Vasopressin 3 Receptor,Antidiuretic Hormone Receptor 1b
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit

The Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit is specifically designed for the precise measurement of AVPR1B levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for various research applications.AVPR1B is a key receptor involved in the regulation of water balance, stress response, and social behavior in mice.

Dysregulation of AVPR1B signaling has been implicated in various physiological and behavioral disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.This ELISA kit is easy to use and delivers accurate results, making it an essential tool for researchers investigating the role of AVPR1B in health and disease in mouse models.

Product Name:Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit
Product Code:MODL00139
Size:96 Assays
Target:AVPR1B
Synonyms:AVPR1-B, AVPR3, Vasopressin 3 Receptor, Antidiuretic Hormone Receptor 1b
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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