Mouse Apoptosis Signaling Pathway Array (17 targets)

Product Sku:	SARB0074
Size:	2, 4, or 8
Species Detected:	Mouse
Number of Targets Detected:	17
Gene Symbols:	AKT1, ATM, BAD, CASP3, CASP7, CDKN1B, CHEK1, EIF2S1, HSPB1, MAP3K7\|MAPK14, MAPK8, NFKBIA, RELA, SMAD2, TP53
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:	Akt Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling / PKC Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
AKT (P-Ser473) Gene Symbol: Akt1 Akt Rac Gene ID: 11651 Accession #: P31750	ATM (P-Ser1981) Gene Symbol: Atm Gene ID: 11920 Accession #: Q62388	BAD (P-Ser112) Gene Symbol: Bad Bbc6 Gene ID: 12015 Accession #: Q61337	Caspase-3 (P-Asp175) Gene Symbol: Casp3 Cpp32 Gene ID: 12367 Accession #: P70677	Caspase-7 (P-Asp198) Gene Symbol: Casp7 Lice2 Mch3 Gene ID: 12369 Accession #: P97864
CHK1 (P-Ser296) Gene Symbol: Chek1 Chk1 Gene ID: 12649 Accession #: O35280	eIF-2a (P-Ser52) Gene Symbol: Eif2s1 Eif2a Gene ID: 13665 Accession #: Q6ZWX6	ERK1/2 (P-Thr202) Gene Symbol: N/A Gene ID: N/A Accession #: Q63844 \| P63085	HSP27 (P-Ser82) Gene Symbol: Hspb1 Hsp25 Hsp27 Gene ID: 15507 Accession #: P14602	IKBa (P-Ser32) Gene Symbol: Nfkbia Ikba Gene ID: 18035 Accession #: Q9Z1E3
JNK (P-Thr183) Gene Symbol: Mapk8 Jnk1 Prkm8 Gene ID: 26419 Accession #: Q91Y86	NF-kB P65 (P-Ser536) Gene Symbol: Rela Nfkb3 Gene ID: 19697 Accession #: Q04207	p27 (P-Thr198) Gene Symbol: Cdkn1b Gene ID: 12576 Accession #: P46414	p38 (P-Thr180/Tyr182) Gene Symbol: Mapk14 Crk1 Csbp1 Csbp2 Gene ID: 26416 Accession #: P47811	P53 (P-Ser15) Gene Symbol: Tp53 P53 Trp53 Gene ID: 22059 Accession #: P02340
SMAD2 (P-Ser245) Gene Symbol: Smad2 Madh2 Madr2 Gene ID: 17126 Accession #: Q62432	TAK1 (P-Ser412) Gene Symbol: Map3k7 Tak1 Gene ID: 26409; Accession #: Q62073

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated anti-IgG
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis