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Mouse Apolipoprotein H (APOH) ELISA Kit

SKU:
MODL00101
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01339
Sensitivity:
1.42ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
Apo-H,B2G1,BG,B2-GP1,B2GP1,Previously,Beta 2 Glycoprotein 1,APC inhibitor,Activated protein C-binding protein,Anticardiolipin cofactor,Beta(2)GPI
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Saliva And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Apolipoprotein H (APOH) ELISA Kit

The Mouse Apolipoprotein H (ApoH) ELISA Kit is specifically designed for the sensitive and accurate detection of apolipoprotein H levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reliable results for various research applications.Apolipoprotein H is an important protein involved in lipid metabolism and blood coagulation regulation. It plays a critical role in maintaining cardiovascular health and has been linked to various diseases such as atherosclerosis and thrombosis.

The Mouse Apolipoprotein H ELISA Kit is essential for studying these conditions and developing potential therapeutic interventions.Overall, this ELISA kit is a valuable tool for researchers seeking to investigate the role of apolipoprotein H in mouse models and explore its implications in cardiovascular and metabolic diseases.

Product Name:Mouse Apolipoprotein H (APOH) ELISA Kit
Product Code:MODL00101
Size:96 Assays
Target:APOH
Synonyms:Apo-H, B2G1, BG, B2-GP1, B2GP1, Previously, Beta 2 Glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Beta(2)GPI
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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