The Mouse ApoB (Apolipoprotein B) ELISA Kit is a cutting-edge assay designed for the precise and quantitative detection of Apolipoprotein B levels in a variety of mouse biological samples. Apolipoprotein B is a key structural protein in several lipoprotein particles, primarily low-density lipoprotein (LDL) known to be a critical player in lipid metabolism and atherosclerosis. As a crucial component of the lipoprotein particles that transport cholesterol in the bloodstream, ApoB has significant implications for cardiovascular health.
Our Mouse ApoB ELISA Kit from Assay Genie is engineered to provide exceptional sensitivity and specificity, ensuring accurate and reproducible results for studies focused on lipid metabolism, cardiovascular diseases, and metabolic disorders. Manufactured under strict quality control standards, the kit offers robust performance and ease of use, making it an indispensable tool for researchers investigating the role of Apolipoprotein B in health and disease.
Product Name:
Mouse ApoB (Apolipoprotein B) ELISA Kit
SKU:
AEES00212
Target:
Mouse ApoB (Apolipoprotein B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoB. You can calculate the concentration of Mouse ApoB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
97-104
101-108
Average (%)
100
101
105
1:4
Range (%)
93-102
95-106
89-103
Average (%)
97
103
96
1:8
Range (%)
97-112
90-96
82-96
Average (%)
104
93
90
1:16
Range (%)
99-106
94-102
101-112
Average (%)
103
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-101
98
EDTA plasma (n=5)
88-98
92
Cell culture media (n=5)
94-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.48
9.02
0.81
2.35
9.9
Standard deviation
0.05
0.11
0.4
0.04
0.11
0.48
C V (%)
5.38
4.44
4.43
4.94
4.68
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoB in samples. No significant cross-reactivity or interference between Mouse ApoB and analogues was observed.
